Transient expression and purification of chimeric heavy chain antibodies

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1016/j.pep.2008.10.011
Author	Search for: Zhang, Jianbing¹; Search for: Liu, Xin¹; Search for: Bell, Andrea¹; Search for: To, Rebecca¹; Search for: Baral, Toya Nath¹; Search for: Azizi, Ali¹; Search for: Li, Jianjun¹; Search for: Cass, Brian²; Search for: Durocher, Yves²
Affiliation	National Research Council of Canada. NRC Institute for Biological Sciences National Research Council of Canada. NRC Biotechnology Research Institute
Format	Text, Article
Subject	(chimeric) heavy chain antibody; antibody expression; single domain antibody; transient expression; biotechnology; in vitro; protein; glycosylation; antibodies, monoclonal; antibody; assay; biosynthesis; camelids, new world; Canada; cell line, tumor; chain; domain; efficient; gene expression; genetics; immunoglobulin heavy chains; monoclonal-antibody; recombinant fusion proteins
Abstract	Monoclonal antibodies have been successfully engineered as approved therapeutics. However, their large size is considered a major factor preventing them from having a more efficient tissue penetration. As the first step to establish a possibly more efficient antibody platform, we present here transient expression, purification and characterization of six chimeric heavy chain antibodies (cHCAbs), or fusion of camelid single domain antibodies (sdAbs) to human fragment crystallizable (Fc). All six HCAbs have a MW of not, vert, similar80 kDa, expressed well in a HEK293 expression system and have G0, G1 and G2 types of glycosylation. The transient expression also provided a very fast way to generate high milligram to low gram amount of proteins for in vitro assays and preliminary animal studies.
Publication date	2008-10-26
In	Protein Expression and Purification 65, no. 1 (26 October 2008): 77–82.
Language	English
Peer reviewed	Yes
NRC number	ZHANG2009A NRCC 47831
NPARC number	15329265
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Record identifier	f0c1afa1-4615-44af-860d-1c805cbb1943
Record created	2010-05-21
Record modified	2020-04-15

Date modified:: 2024-04-20