Analysis of the promoter region of the gene LIP1 encoding triglyceride lipase from Fusarium graminearum

  1. Get@NRC: Analysis of the promoter region of the gene LIP1 encoding triglyceride lipase from Fusarium graminearum (Opens in a new window)
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Journal titleMicrobiological Research
Pages618628; # of pages: 11
SubjectBasal expression; Cis-acting element; Cis-regulatory elements; Deletion mutants; Flanking sequence; Fusarium graminearum; Fusarium head blights; Gene induction; Gene regulations; GFP expression; Green fluorescent protein; In-silico; Long-chain fatty acids; Open reading frame; Promoter activities; Promoter region; Promoter sequences; Regulatory elements; Encoding (symbols); Fluorescence; Gene encoding; Gene expression; Glucose; Glycerol; Lipases; Fatty acids; green fluorescent protein; triacylglycerol lipase; alcohol; biodegradation; catalysis; enzyme activity; ester; fatty acid; fluorescence; fungus; gene expression; mutation; pathogen; article; binding site; enzymology; Fusarium; gene deletion; gene fusion; genetics; metabolism; regulatory sequence; reporter gene; wheat; Artificial Gene Fusion; Binding Sites; Fusarium; Genes, Reporter; Green Fluorescent Proteins; Lipase; Regulatory Sequences, Nucleic Acid; Sequence Deletion; Triticum; Fusarium; Gibberella zeae; Triticum aestivum
AbstractTriglyceride lipases catalyze the reversible degradation of glycerol esters with long-chain fatty acids into fatty acids and glycerol. In silico analysis of 5'-end flanking sequence of the gene LIP1 encoding a triglyceride lipase from the wheat head blight pathogen Fusarium graminearum revealed the presence of several cis-regulatory elements. To delineate the function of these regulatory elements, we constructed a series of deletion mutants in the LIP1 promoter region fused to the open reading frame of a green fluorescent protein (GFP) and assayed the promoter activity. Analysis of GFP expression levels in mutants indicated that a 563-bp promoter sequence was sufficient to drive the expression of LIP1 and regulatory elements responsible for the gene induction were located within the 563-372. bp region. To further investigate the regulatory elements, putative cis-acting elements spanned within the 563-372. bp region were mutated using a targeted mutagenesis approach. A CCAAT box, a CreA binding site, and a fatty acid responsive element (FARE) were identified and confirmed to be required for the basal expression of LIP1, glucose suppression and fatty acid induction, respectively. © 2011 Elsevier GmbH.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Plant Biotechnology Institute (PBI-IBP)
Peer reviewedYes
NPARC number21271088
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Record identifierefb3b093-9a19-42a5-8aeb-50d33c9081c8
Record created2014-03-24
Record modified2016-05-09
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