Introducing a new bioengineered bug: Methylobacterium extorquens tuned as a microbial bioplastic factory

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Journal titleBioengineered Bugs
Pages7179; # of pages: 9
SubjectAcids; activity; Acyl-CoA desaturase; Bacteria; bio; biosynthesis; Biotechnology; Canada; Carbon; Enzymes; Escherichia coli; Fatty Acids; Functionalized polyhydroxyalkanoates; Funtionalized polyhydroxyalkanoates; Genes; Genetic Engineering; genome; Genome sequence; Hydrocarbons; Hydrolysis; Isomerase-dependent beta-oxidation; Liver; metabolism; Methane; methane oxidation; Methanol; Methylobacterium extorquens; microbiology; Mitochondria; Mutagenesis; pha; plastic; Plastics; Protein; Purification; rat; Reductase-dependent beta-oxidation; Scopus; Temperature; Unsaturated fatty acids; Yeast
AbstractDiscussion on and use of methanol as chemical feedstock and as alternative fuel has gained momentum during the past years. Consequently, microorganism and product design based on "methylotrophism" is in vogue as reflected by increasing research and development activities in methanolrelated areas. A recent example of microorganism and product development is the use of recombinant Methylobacterium extorquens ATCC 55366 strains in the production of second generation biopolyesters. Feeding n-alkenoic acids in addition to methanol yielded functionalized polyhydroxyalkanoates (PHAs) and uncovered how M. extorquens copes with fatty acids. While some parts of the degradation pathway remain unclear, possible metabolic routes are suggested that may explain the significant loss of double bonds prior to polymerization of PHA precursors and occurrence of oddnumbered monomers derived from evennumbered n-alkenoic acids. In addition, microbial discoloration upon fatty acid feeding is discussed and future directions for further genetic modification of M. extorquens are provided. -¬ 2011 Landes
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC number52781
NPARC number18335884
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Record identifieredbf619e-4a8c-41e7-bad0-51bbf04a78a1
Record created2011-07-28
Record modified2017-03-23
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