Download | - View accepted manuscript: Study of chronic lymphocytic leukemia cells by FT-IR spectroscopy and cluster analysis (PDF, 1.4 MiB)
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DOI | Resolve DOI: https://doi.org/10.1016/0145-2126(96)00019-7 |
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Author | Search for: Schultz, Christian P.1; Search for: Liu, Kan-Zhi1; Search for: Johnston, James B.; Search for: Mantsch, Henry H.1 |
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Affiliation | - National Research Council of Canada. NRC Institute for Biodiagnostics
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Format | Text, Article |
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Subject | FT-IR spectroscopy; chronic lymphocytic leukemia; cluster analysis; B-Lymphocyte; Fourier spectrometry; lipids; proteins; chronic; malignant hemopathy; Lymphoproliferative Syndrome; spectrométrie de FT-IR; analyse amas; lymphocyte B; spectrométrie Fourier; lipide; protéine; chronique; hémopathie maligne; Syndrome lymphoprolifératif |
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Abstract | The peripheral mononuclear cells from 23 normal individuals and the purified B cells from 38 patients with chronic lymphocytic leukemia (CLL) were examined by Fourier transform infrared (FT-IR) spectroscopy. Differences were observed between the CLL and normal cells at the DMA, protein and lipid levels, with CLL cells having greater DNA and lower lipid contents than normal cells. In addition, the spectral character of the CLL and normal cells varied, demonstrating that there were also qualitative differences in the DNA and lipids. Statistical analysis, based on hierarchical clustering, separated normal from CLL cells completely and classified them into two subgroups for normal cells, while the CLL cells could be divided into three subgroups that were distinct from the normal cells. These differences were based on the lipid and DNA content and the overall spectral character of the cells. |
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Publication date | 1996-08 |
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Terms of use | - You may reproduce (print, make photocopies, or download) the accepted manuscript without permission for non-commercial purposes (research, education, and private study), on the condition that you provide proper attribution of the source in all copies.
- Vous pouvez reproduire (imprimer, faire des photocopies ou télécharger) le manuscrit accepté sans autorisation à des fins non commerciales (recherche, éducation et études privées), à condition de fournir la source appropriée dans toutes les copies.
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Access condition | |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NRC number | 415 |
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NPARC number | 9201851 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | ed05b69f-f63c-4e6a-a077-25b97a2781e5 |
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Record created | 2009-10-02 |
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Record modified | 2020-03-20 |
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