In-storage psychrophilic anaerobic digestion: acclimated microbial kinetics

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Journal titleEnvironmental Technology
Pages17631772; # of pages: 10
Subjectpsychrophilic anaerobic digestion, specific substrate uptake, manure treatment, microbial kinetics, swine manure
AbstractIn-storage psychrophilic anaerobic digestion develops by microbial acclimation in covered swine-manure storage tanks, producing CH4 and stabilizing organic matter. To optimize the system's performance, the process kinetics must be understood. The objective of this study was to evaluate kinetic parameters describing the major stages in the digestion process, and to investigate the effect of temperature acclimation on these parameters. Specific activity tests were performed using manure inocula and five substrates at three incubation temperatures. Extant substrate activities were determined analytically for each case, and intrinsic kinetic parameters for glucose uptake were estimated by grid search fitting to the Monod model. The results demonstrate that this acclimated microbial community exhibits different kinetic parameters to those of the mesophilic communities currently modelled in the literature, with increased activity at low temperatures, varying with substrate and temperature. For glucose, the higher uptake is accompanied by lower microbial yield and half-saturation constant. Decomposing these values suggests that active psychrophilic and mesophilic microbial populations co-exist within the community. This work also confirms that a new method of assessing microbial substrate kinetics must be developed for manure microbial communities, separating microbial mass from other suspended organics.
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NPARC number21269105
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Record identifierea0a3824-1f41-415d-9242-63a682772846
Record created2013-12-05
Record modified2016-05-09
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