Validation of a high-performance liquid chromatographic assay for the quantification of Reovirus particles type 3

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Pages417421; # of pages: 5
SubjectAnimal; bio; biotechnology; chromatography; human; reovirus; oncolytics; HPLC; quantification; validation
AbstractAn anion exhange high-performance liquid chromatography (HPLC) method for the quantification of human Reovirus type 3 particles was validated according to the performance criteria of precision, specificity, linearity of calibration and working range, limits of detection and quantification, accuracy and recovery. Samples taken at various stages of Reovirus purification were used for the validation of the method. The method was specific for Reovirus which eluted around 9.8 min without interference from any other component in the sample. Reovirus can be detected between 0.32E+12 and 2.10E12 VP/mL by the proposed method that has the correlation coefficient of linearity equal to 0.9974 and the slope of linearity equal to 5,74E-07 area units/(VP mL).
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
NoteDA - 20071023IS - 0731-7085 (Print)LA - engPT - Journal ArticleSB - IM
Peer reviewedNo
NRC number47812
NPARC number3539542
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Record identifiere0665e93-5428-46d7-822d-e71bd126ca08
Record created2009-03-01
Record modified2016-05-09
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