Degradation of CL-20 by white-rot fungi

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Journal titleChemosphere
Pages175181; # of pages: 7
AbstractIn previous studies, we found that the emerging energetic chemical, CL-20 (C6H6N12O12, 2,4,6,8,10,12-hexanitro-2,4,6,8,10,12-hexaazaisowurtzitane), can be degraded following its initial denitration using both aerobic and anaerobic bacteria. The C and N mass balances were not determined due to the absence of labeled starting compounds. The present study describes the degradation of the emerging contaminant by Phanerochaete chrysosporium using ring-labeled [15N]-CL-20 and [14C]-CL-20. Ligninolytic cultures degraded CL-20 with the release of nitrous oxide (N2O) in amounts corresponding to 45% of the nitrogen content of CL-20. When ring-labeled [15N]-CL-20 was used, both 14N14NO and 15N14NO were observed, likely produced from -NO2 and N-NO2, respectively. The incubation of uniformly labeled [14C]-CL-20 with fungi led to the production of 14CO2 (>80%). Another ligninolytic fungus, Irpex lacteus, was also able to degrade CL-20, but as for P. chrysosporium, no early intermediates were observed. When CL-20 was incubated with manganese peroxidase (MnP), we detected an intermediate with a [M-H]- mass ion at 345 Da (or 351 and 349 Da when using ring-labeled and nitro-labeled [15N]-CL-20, respectively) matching a molecular formula of C6H6N10O8. The intermediate was thus tentatively identified as a doubly denitrated CL-20 product. The concomitant release of nitrite ions (Click to view the MathML source) with CL-20 degradation by MnP also supported the occurrence of an initial denitration prior to cleavage and decomposition.Keywords: CL-20; Degradation; Nitramine; Phanerochaete chrysosporium; Fungi
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AffiliationNational Research Council Canada; NRC Biotechnology Research Institute
Peer reviewedNo
NRC number45920
NPARC number3538764
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Record identifierdf50906a-1d06-4672-ad99-063093d58a06
Record created2009-03-01
Record modified2016-05-09
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