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Stability of pressure-extruded liposomes made from archaeobacterial ether lipids

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/BF00902745
AuthorSearch for: 1; Search for: 1; Search for: ; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Institute for Biological Sciences
FormatText, Article
Abstract
Ether lipids were obtained from a wide range of archaeobacteria grown at extremes of pH, temperature, and salt concentration. With the exception ofSulfolobus acidocaldarius, unilamellar and/or multilamellar liposomes could be prepared from emulsions of total polar lipid extracts by pressure extrusion through filters of various pore sizes. Dynamic light scattering, and electron microscopy revealed homogeneous liposome populations with sizes varying from 40 to 230 nm, depending on both the lipid source and the pore size of the filters. Leakage rates of entrapped fluorescent or radioactive compounds established that those archaeobacterial liposomes that contained tetraether lipids were the most stable to high temperatures, alkaline pH, and serum proteins. Most ether liposomes were stable to phospholipase A2, phospholipase B and pancreatic lipase. These properties of archaeobacterial liposomes make them attractive for applications in biotechnology.
Publication date
In
  • Applied Microbiology and Biotechnology 42, no. 2-3 (1994): 375–384.
LanguageEnglish
NRC numberCHOQUET1994B
NRCC 37391
NPARC number9382920
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Record created2009-07-10
Record modified2020-04-27
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