Identification and characterization of hepsin/-TM, a non-transmembrane hepsin isoform

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Pages157165; # of pages: 9
SubjectAdenocarcino; Brain; Cell; e; Huma; Kidne; Live; ne; E; oteas; pha; ymes; gen
AbstractType II transmembrane serine proteases (TTSPs), including hepsin, are a new class of cell surface catalytic enzymes. In the present study, a non -transmembrane isoform of hepsin, named hepsin/-TM that originates from alternative splicing, was identified. Unlike the transmembrane hepsin isoform, this non-transmembrane isoform was distributed within the cytoplasm. Real-time PCR experiments revealed that while hepsin was expressed in all tested human tissues, hepsin/-TM was restricted in kidney, brain and lung tissues. Significantly, hepsin/-TM was not expressed in liver where hepsin was originally identified. However, hepsin/-TM was highly expressed in brain where hepsin was expressed at a relatively lower level. Moreover, these two isoforms showed different expression patterns in a number of colon adenocarcinoma cell lines. In addition, in contrast to hepsin, expression of hepsin/-TM in vivo does not exert any apparent inhibitory effect on mammalian cell growth
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number46235
NPARC number3540187
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Record identifierd91b67d6-c077-41f5-8348-4538c87a13de
Record created2009-03-01
Record modified2016-05-09
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