| Author | Search for: Steiner, K.; Search for: Novotny, R.; Search for: Patel, K.; Search for: Vinogradov, Evguenii; Search for: Whitfield, C.; Search for: Valvano, M.; Search for: Messner, P.; Search for: Schaffer, C. |
|---|
| Format | Text, Article |
|---|
| Subject | Amino Acid Sequence; Bacillaceae; Bacteria; bacterial; Bacterial Proteins; biosynthesis; Carbohydrate Sequence; CHAIN; chemistry; CLUSTER; Conserved Sequence; ENZYME; Escherichia; Escherichia coli; ESCHERICHIA-COLI; Galactosyltransferases; GENE; genetics; GLYCAN CHAIN; glycoprotein; Glycoproteins; Glycosylation; In Vitro; Linker; Lipopolysaccharides; membrane; Membrane Glycoproteins; metabolism; Molecular Sequence Data; Plasmids; POLYSACCHARIDE; Polysaccharides; protein; Protein Conformation; REPEATING UNIT; RESIDUES; Salmonella; Sequence Alignment; Sequence Homology,Amino Acid; STRAIN; STRAINS; surface; transfer; transferase; TRISACCHARIDE; UNIT |
|---|
| Abstract | The glycan chain of the S-layer glycoprotein of Geobacillus stearothermophilus NRS 2004/3a is composed of repeating units [-->2)-alpha-l-Rhap-(1-->3)-beta-l-Rhap-(1-->2)-alpha-l-Rhap-(1-->], with a 2-O-methyl modification of the terminal trisaccharide at the nonreducing end of the glycan chain, a core saccharide composed of two or three alpha-l-rhamnose residues, and a beta-d-galactose residue as a linker to the S-layer protein. In this study, we report the biochemical characterization of WsaP of the S-layer glycosylation gene cluster as a UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase that primes the S-layer glycoprotein glycan biosynthesis of Geobacillus stearothermophilus NRS 2004/3a. Our results demonstrate that the enzyme transfers in vitro a galactose-1-phosphate from UDP-galactose to endogenous phosphoryl-polyprenol and that the C-terminal half of WsaP carries the galactosyltransferase function, as already observed for the UDP-Gal:phosphoryl-polyprenol Gal-1-phosphate transferase WbaP from Salmonella enterica. To confirm the function of the enzyme, we show that WsaP is capable of reconstituting polysaccharide biosynthesis in WbaP-deficient strains of Escherichia coli and Salmonella enterica serovar Typhimurium |
|---|
| Publication date | 2007-04 |
|---|
| In | |
|---|
| Language | English |
|---|
| NRC number | STEINER2007 |
|---|
| NPARC number | 9364325 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | d6804f6f-3573-4485-b800-2872911318b9 |
|---|
| Record created | 2009-07-10 |
|---|
| Record modified | 2022-11-18 |
|---|
