Overview of current scalable methods for purification of viral vectors

DOIResolve DOI: http://doi.org/10.1007/978-1-61779-095-9_4
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TypeBook Chapter
Book titleViral Vectors for Gene Therapy : Methods and Protocols
Series titleMethods in Molecular Biology; Volume 737
Pages89116; # of pages: 28
Subjectviral vectors; gene therapy; purification; downstream processing; centrifugation; membrane filtration; chromatography
AbstractAs a result of the growing interest in the use of viruses for gene therapy and vaccines, many virus-based products are being developed. The manufacturing of viruses poses new challenges for process developers and regulating authorities that need to be addressed to ensure quality, efficacy, and safety of the final product. The design of suitable purification strategies will depend on a multitude of variables including the vector production system and the nature of the virus. In this chapter, we provide an overview of the most commonly used purification methods for viral gene therapy vectors. Current chromatography options available for large-scale purification of γ-retrovirus, lentivirus, adenovirus, adeno-associated virus, herpes simplex virus, baculovirus, and poxvirus vectors are presented.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC numberCPBIO19
NPARC number21268259
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Record identifierd5305573-3f1b-4720-96bb-bcf64e54c165
Record created2013-06-11
Record modified2016-05-09
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