| Download | - View accepted manuscript: The Structural Variety of DNA-DPPC-Divalent Metal Cation Aggregates: SAXD and SANS Study (PDF, 841 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1140/epjst/e2009-00957-0 |
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| Author | Search for: Uhr´ıkov´a, D.; Search for: Pullmannov´a, P.; Search for: Kuˇcerka, N.1; Search for: Funari, S.S.; Search for: Teixeira, J.; Search for: Balgav´y, P. |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Abstract | We examine the structure of aggregates formed due to DNA interaction with dipalmitoylphosphatidylcholine (DPPC) in presence of Ca2+ and Zn2+ using small-angle synchrotron X-ray diffraction (SAXD) and neutron scattering (SANS). SAXD shows structural heterogeneity as a function of the cation concentration and temperature: At low cation concentration (∼1mM), aggregates show two DPPC phases, one with a lateral segregation of DNA and cation, while higher cation concentration improves the DNA packing and the condensed lamellar phase is observed in DNA + DPPC + 20mMion2+ aggregates. The SANS detected the dissolution of the condensed lamellar phase into unilamellar DPPC + Zn2+ vesicles due to gel → liquid-crystal phase transition in DNA + DPPC + 20mM Zn2+ aggregates with the short fragmented salmon sperm DNA. |
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| Publication date | 2009-06-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 14829324 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | ca0d3cbf-d6e6-434c-b972-b6556712b6fe |
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| Record created | 2011-02-15 |
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| Record modified | 2020-04-16 |
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