Alternative title | Structure of non-activated Pisum sativum Rubisco in complex with its substrate ribulose-1,5-bisphosphate |
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Download | - View accepted manuscript: Structure of Pisum sativum Rubisco with bound ribulose 1,5-bisphosphate (PDF, 1.5 MiB)
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DOI | Resolve DOI: https://doi.org/10.1107/S1744309112047549 |
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Author | Search for: Loewen, Peter C.; Search for: Didychuk, Allison L.1; Search for: Switala, Jacek; Search for: Perez-Luque, Rosa; Search for: Fita, Ignacio; Search for: Loewen, Michele C.1 |
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Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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Format | Text, Article |
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Subject | molecular replacement; Pisum sativum; ribulose 1,5-bisphosphate; Rubisco |
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Abstract | The first structure of a ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) from a pulse crop is reported. Rubisco was purified from Pisum sativum (garden pea) and diffraction-quality crystals were obtained by hanging-drop vapour diffusion in the presence of the substrate ribulose 1,5-bisphosphate. X-ray diffraction data were recorded to 2.20 Å resolution from a single crystal at the Canadian Light Source. The overall quaternary structure of non-activated P. sativum Rubisco highlights the conservation of the form I Rubisco hexadecameric complex. The electron density places the substrate in the active site at the interface of the large-subunit dimers. Lys201 in the active site is not carbamylated as expected for this non-activated structure. Some heterogeneity in the small-subunit sequence is noted, as well as possible variations in the conformation and contacts of ribulose 1,5-bisphosphate in the large-subunit active sites. Overall, the active-site conformation most closely correlates with the `closed' conformation observed in other substrate/inhibitor-bound Rubisco structures. |
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Publication date | 2013-01 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NRC number | NRCC 55421 |
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NPARC number | 21268467 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | c8dc6913-970d-4981-9cfc-661968ce8fac |
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Record created | 2013-07-24 |
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Record modified | 2020-06-04 |
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