Overexpression of a FAD3 desaturase increases synthesis of a polymethylene-interrupted dienoic fatty acid in seeds of Arabidopsis thaliana L.

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Download	View accepted manuscript: Overexpression of a FAD3 desaturase increases synthesis of a polymethylene-interrupted dienoic fatty acid in seeds of Arabidopsis thaliana L. (PDF, 1.2 MiB)
DOI	Resolve DOI: https://doi.org/10.1007/s11745-009-3315-5
Author	Search for: Puttick, Debbie¹; Search for: Dauk, Melanie; Search for: Lozinsky, Sharla¹; Search for: Smith, Mark A.
Affiliation	National Research Council of Canada. NRC Plant Biotechnology Institute
Format	Text, Article
Subject	Linoleate desaturase; Mangiferic acid; Polymethylene-interrupted dienoic fatty acid; Arabidopsis; Seed oil
Abstract	A cDNA encoding the Arabidopsis extraplastidic linoleate desaturase (FAD3) was overexpressed in the seeds of wild-type Arabidopsis and in a mutant line that accumulates high levels of oleic acid. In the transformed wild-type plants, linolenic acid (18:3Δ9,12,15) increased from 19% to nearly 40% of total seed fatty acids, with a corresponding decrease in linoleate content (18:2Δ9,12). In the high oleate mutant, a large increase in the level of a fatty acid identified by gas-chromatography/mass-spectrometry as mangiferic acid (18:2Δ9,15) was observed. The results demonstrate that the polymethylene-interrupted dienoic fatty acid, mangiferic acid, can be produced in seed oil through the overexpression of a fatty acid n-3 desaturase.
Publication date	2009-06-23
In	Lipids 44, no. 8 (23 June 2009): 753–757.
Language	English
Peer reviewed	Yes
NRC number	NRCC 50144
NPARC number	19078543
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Record identifier	c82b7e64-88c2-4831-b989-66ec2d59b778
Record created	2012-03-30
Record modified	2020-04-16

Date modified:: 2024-04-20