Reinvestigation of the structure of Brucella O-antigens

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Journal titleCarbohydrate Research
Pages144147; # of pages: 4
Subject2-D NMR; Brucella; LPS; MS; O-Chain; O-specific polysaccharides; Pentasaccharides; Tetrasaccharides; Nuclear magnetic resonance; Polymers; Strain; Structure (composition); Epitopes; epitope; O antigen; pentasaccharide; tetrasaccharide; acetylation; antigen structure; article; Brucella melitensis; carbon nuclear magnetic resonance; deacylation; nonhuman; priority journal; proton nuclear magnetic resonance; Brucella; Brucella melitensis; Brucella melitensis 16M
AbstractO-Specific polysaccharides of Brucella contain two antigenic determinants, called A and M. Most of the strains express epitope A with a small amount of epitope M, whereas Brucella melitensis strain 16 M expresses longer polymer consisting mostly of M-type epitopes. Proposed explanation was that epitope A is defined by 1-2-linked homopolymer of N-formylperosamine (Rha4NFo), while epitope M is a pentasaccharide with four 2- and one 3-substituted Rha4NFo. We reinvestigated both types of structures by 2D NMR and showed that M-epitope is a tetrasaccharide, missing one of the 2-linked Rha4NFo as compared to the previously proposed structure. Polysaccharide from B. melitensis 16 M contains a fragment of 1-2-linked polymer, capped with M-type polymer. Other strains contain one or two M-type units at the non-reducing end of the 1-2-linked O-chain. Crown Copyright © 2013 Published by Elsevier Ltd.
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AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21270650
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Record identifierc6f9a02c-a976-4c8a-a40a-f32fb9bd0dc1
Record created2014-02-17
Record modified2016-05-09
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