| DOI | Resolve DOI: https://doi.org/10.1016/j.bbamem.2010.11.015 |
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| Author | Search for: Keating, Eleonora; Search for: Waring, Alan J.; Search for: Walther, Frans J.; Search for: Possmayer, Fred; Search for: Veldhuizen, Ruud A. W.; Search for: Petersen, Nils O.1 |
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| Affiliation | - National Research Council of Canada. National Institute for Nanotechnology
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| Format | Text, Article |
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| Subject | cholesterol; lung surfactant; surfactant function; peptide; phospholipid; Langmuir Blodgett film; atomic force microscopy; phase separation; protein function; protein lipid interaction; protein localization; surface property; time of flight secondary mass spectrometry; lipid bilayers; pulmonary alveoli; pulmonary surfactants; surface tension; lipids proteins lateral organization |
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| Abstract | Pulmonary surfactant is a complex lipid-protein mixture whose main function is to reduce the surface tension at the air-liquid interface of alveoli to minimize the work of breathing. The exact mechanism by which surfactant monolayers and multilayers are formed and how they lower surface tension to very low values during lateral compression remains uncertain. We used time-of-flight secondary ion mass spectrometry to study the lateral organization of lipids and peptide in surfactant preparations ranging in complexity. We show that we can successfully determine the location of phospholipids, cholesterol and a peptide in surfactant Langmuir-Blodgett films and we can determine the effect of cholesterol and peptide addition. A thorough understanding of the lateral organization of PS interfacial films will aid in our understanding of the role of each component as well as different lipid-lipid and lipid-protein interactions. This may further our understanding of pulmonary surfactant function. © 2010 Elsevier B.V. All rights reserved. |
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| Publication date | 2010-11-24 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21271896 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | c6f6496a-ddca-4ce0-906c-945b2c7c19c4 |
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| Record created | 2014-05-05 |
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| Record modified | 2020-04-17 |
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