Bioremediation of highly energetic compounds: a search for remediation technologies

AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for: ; Search for:
Proceedings titleWater Science & Technology
ConferenceInternational Conference on Waste Minimisation and End of Pipe Treatment in Chemical and Petrochemical Industries (IAWQ 1999), Nov. 14-18, 1999, Yucatan, Mexico
Pages385393; # of pages: 9
Subjectcrossgroup; bioremediation; explosives; intermediates; mineralization; P. chrysosporium; Rhodococcus; sludge
AbstractWhen TNT (N-source) was treated with anaerobic sludge it biotransformed into triaminotoluene (TAT) in high yield (80%). Results of experiments using ¹³C-labeling indicate that denitrated or deaminated products such as p-cresol and toluene were not formed. Whereas ¹⁴C-labeling showed negligible mineralization (<0.1% 14CO₂) despite the complete disappearance of TNT. On the other hand, when TNT (175μM) was treated with the fungus Phanerochaete chrysosporium it disappeared completely in less than two weeks, but mineralization (liberated ¹⁴CO₂) did not exceed 1%. Several intermediates, marked with the initial formation of the two monohydroxylamino-dinitrotoluene (HADNT) followed by their transformation to monoamino-dinitrotoluenes (ADNT), diamines (DANT), acetylated TNT products, and azo and hydrazo derivatives were detected. In contrast, high concentrations (ca 20,000 ppm) of RDX and HMX were effectively degraded (ca 70%) in soil slurries using municipal anaerobic sludge. RDX and HMX disappearance was accompanied by the elimination of toxicity associated with RDX and HMX as determined by the Microtox test.
Publication date
AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedYes
NRC numberCPENV20
NPARC number3539845
Export citationExport as RIS
Report a correctionReport a correction
Record identifierc39c27a4-ce49-4cfc-8ee2-29d53f1c9a66
Record created2009-03-01
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: