Methods and applications of serological proteome analysis

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1007/978-1-62703-589-7_5
Author	Search for: Fulton, K.M.¹; Search for: Martin, S.S.; Search for: Wolfraim, L.; Search for: Twine, S.M.¹
Affiliation	National Research Council of Canada. Human Health Therapeutics
Format	Text, Article
Subject	proteome; tularemia vaccine; blood analysis; immunoblotting; immunoreactivity; isoelectric focusing; mouse; polyacrylamide gel electrophoresis; protein analysis; serological proteome analysis; serology; two dimensional gel electrophoresis; vaccination; Western blotting
Abstract	The study of the humoral response to infectious diseases and chronic diseases, such as cancer, is important for many reasons, including understanding the host response to disease, identification of protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. During the past decade, proteomic approaches, such as serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SERPA approach, using the analysis of sera from mice vaccinated with a live attenuated tularemia vaccine as an example. © 2013 Springer Science+Business Media, LLC.
Publication date	2013
In	Methods in Molecular Biology 1061 (2013): 97–112.
Language	English
Peer reviewed	Yes
NPARC number	21269763
Export citation	Export as RIS
Report a correction	Report a correction (opens in a new tab)
Record identifier	c1a20eb1-f7aa-4d65-b03c-c6f930aef793
Record created	2013-12-13
Record modified	2020-04-22

Date modified:: 2024-04-19