Methods and applications of serological proteome analysis

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Journal titleMethods in Molecular Biology
Pages97112; # of pages: 16
Subjectproteome; tularemia vaccine; article; blood analysis; immunoblotting; immunoreactivity; isoelectric focusing; mouse; nonhuman; polyacrylamide gel electrophoresis; priority journal; protein analysis; serological proteome analysis; serology; two dimensional gel electrophoresis; vaccination; Western blotting
AbstractThe study of the humoral response to infectious diseases and chronic diseases, such as cancer, is important for many reasons, including understanding the host response to disease, identification of protective antigens, vaccine development, and discovery of biomarkers for early diagnosis. During the past decade, proteomic approaches, such as serological proteome analysis (SERPA), have been used to identify the repertoire of immunoreactive proteins in various diseases. In this chapter, we provide an outline of the SERPA approach, using the analysis of sera from mice vaccinated with a live attenuated tularemia vaccine as an example. © 2013 Springer Science+Business Media, LLC.
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AffiliationNational Research Council Canada (NRC-CNRC); Human Health Therapeutics (HHT-TSH)
Peer reviewedYes
NPARC number21269763
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Record identifierc1a20eb1-f7aa-4d65-b03c-c6f930aef793
Record created2013-12-13
Record modified2016-05-09
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