| Download | - View accepted manuscript: Single-Domain Antibody Bioconjugated Near-IR Quantum Dots for Targeted Cellular Imaging of Pancreatic Cancer (PDF, 1019 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1166/jnn.2011.4167 |
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| Author | Search for: Zaman, Md. Badruz1; Search for: Baral, Toya Nath1; Search for: Jakubek, Zygmunt J.2; Search for: Zhang, Jianbing1; Search for: Wu, Xiaohua3; Search for: Lai, Edward; Search for: Whitfield, Dennis1; Search for: Yu, Kui2 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
- National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
- National Research Council of Canada. NRC Institute for Microstructural Sciences
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| Format | Text, Article |
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| Subject | Photoluminescent Near-IR Quantum Dots; Single-Domain Antibody; In Vitro Targeted Cellular Imaging; Colloidal Nanocrystals; Pancreatic and Breast Cancer |
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| Abstract | Successful targeted imaging of BxPC3 human pancreatic cancer cells is feasible with near-IR CdTeSe/CdS quantum dots (QDs) functionalized with single-domain antibody (sdAb) 2A3. For specific targeting, sdAbs are superior to conventional antibodies, especially in terms of stability, aggregation, and production cost. The bright CdTeSe/CdS QDs were synthesized to emit in the diagnostic window of 650-900 nm with a narrow emission band. 2A3 was derived from llama and is small in size of ∼13 kDa, but with fully-functional recognition to the target carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6), a possible biomarker as a therapeutic target of pancreatic cancer. For compelling imaging, optical may be the most sensible among the various imaging modalities, regarding the sensitivity and cost. This first report on sdAb-conjugated near-IR QDs with high signal to background sensitivity for targeted cellular imaging brings insights into the development of optical molecular imaging for early stage cancer diagnosis. |
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| Publication date | 2011-01-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 17652983 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | bc8a1ff6-0b8b-4268-a243-69ff2cef5943 |
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| Record created | 2011-03-31 |
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| Record modified | 2020-04-21 |
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