| Download | - View accepted manuscript: Label-free imaging of arterial tissues using photonic crystal fibre (PCF) based nonlinear optical microscopic system (PDF, 865 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1117/12.809361 |
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| Author | Search for: Ko, Alex C. T.1; Search for: Ridsdale, Andrew2; Search for: Pegoraro, Adrian F.2; Search for: Smith, Michael S. D.1; Search for: Mostaço-Guidolin, Leila B.; Search for: Hewko, Mark D.1; Search for: Kohlenberg, Elicia M.1; Search for: Schattka, Bernie J.1; Search for: Shiomi, Masashi; Search for: Stolow, Albert2; Search for: Sowa, Michael G.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biodiagnostics
- National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
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| Format | Text, Article |
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| Conference | Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues VII, 26 January 2009, San Jose, CA, USA |
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| Subject | Nonlinear optical microscopy; photonic crystal fibre; coherent anti-Stokes Raman scattering; two photon excited fluorescence; second harmonic generation; Atherosclerosis |
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| Abstract | Nonlinear optical (NLO) microscopy provides a minimally invasive optical method for fast molecular imaging at subcellular resolution with 3D sectioning capability in thick, highly scattering biological tissues. In the current study, we demonstrate the imaging of arterial tissue using a nonlinear optical microscope based on photonic crystal fiber and a single femto-second oscillator operating at 800nm. This NLO microscope system is capable of simultaneous imaging extracellular elastin/collagen structures and lipid distribution within aortic tissue obtained from coronary atherosclerosis-prone WHHLMI rabbits (Watanabe heritable hyperlipidemic rabbit-myocardial infarction) Clear pathological differences in arterial lumen surface were observed between healthy arterial tissue and atherosclerotic lesions through NLO imaging. |
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| Publication date | 2009-02-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 19697993 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | bb462be8-6197-47c9-af3e-09cc07c8a5b3 |
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| Record created | 2012-03-22 |
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| Record modified | 2020-04-16 |
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