Phosphorylcholine allows for evasion of bactericidal antibody by haemophilus influenzae

  1. Get@NRC: Phosphorylcholine allows for evasion of bactericidal antibody by haemophilus influenzae (Opens in a new window)
DOIResolve DOI:
AuthorSearch for: ; Search for: ; Search for: ; Search for: ; Search for:
Journal titlePLoS Pathogens
Article numbere1002521
Subjectbacterium antibody; lipopolysaccharide; phosphorylcholine; trypsin; lipopolysaccharide; adaptive immunity; animal experiment; antigen binding; article; bacterial colonization; bactericidal activity; cell surface; controlled study; flow cytometry; Haemophilus influenzae; mouse; nasopharynx; nonhuman; outer membrane; protein expression; survival; adaptive immunity; animal; antibody combining site; Bagg albino mouse; cell membrane; host pathogen interaction; human; immune evasion; immunology; mouse mutant; pathogenicity; Haemophilus influenzae; Murinae; Adaptive Immunity; Animals; Binding Sites, Antibody; Cell Membrane; Haemophilus influenzae; Host-Pathogen Interactions; Humans; Immune Evasion; Lipopolysaccharides; Mice; Mice, Inbred BALB C; Mice, SCID; Phosphorylcholine
AbstractThe human pathogen Haemophilus influenzae has the ability to quickly adapt to different host environments through phase variation of multiple structures on its lipooligosaccharide (LPS), including phosphorylcholine (ChoP). During colonization with H. influenzae, there is a selection for ChoP+ phase variants. In a murine model of nasopharyngeal colonization, this selection is lost in the absence of adaptive immunity. Based on previous data highlighting the importance of natural antibody in limiting H. influenzae colonization, the effect of ChoP expression on antibody binding and its bactericidal activity was investigated. Flow cytometric analysis revealed that ChoP+ phase variants had decreased binding of antibody to LPS epitopes compared to ChoP- phase variants. This difference in antibody binding correlated with increased survival of ChoP+ phase variants in the presence of antibody-dependent, complement-mediated killing. ChoP+ phase variants were also more resistant to trypsin digestion, suggesting a general effect on the physical properties of the outer membrane. Moreover, ChoP-mediated protection against antibody binding correlated with increased resilience of outer membrane integrity. Collectively, these data suggest that ChoP expression provides a selective advantage during colonization through ChoP-mediated effects on the accessibility of bactericidal antibody to the cell surface. © 2012 Clark et al.
Publication date
AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Biological Sciences (IBS-ISB)
Peer reviewedYes
NPARC number21269315
Export citationExport as RIS
Report a correctionReport a correction
Record identifierb548c2de-8239-4c2d-a9f9-bca2b35eb461
Record created2013-12-12
Record modified2016-05-09
Bookmark and share
  • Share this page with Facebook (Opens in a new window)
  • Share this page with Twitter (Opens in a new window)
  • Share this page with Google+ (Opens in a new window)
  • Share this page with Delicious (Opens in a new window)
Date modified: