Comparative assessment of next-generation sequencing, denaturing gradient gel electrophoresis, clonal restriction fragment length polymorphism and cloning-sequencing as methods for characterizing commercial microbial consortia

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1016/j.mimet.2014.11.013
AuthorSearch for: ; Search for: ; Search for: 1; Search for: ; Search for: ; Search for:
Affiliation
  1. National Research Council of Canada. Human Health Therapeutics
FormatText, Article
SubjectDNA 16S; hydrocarbon; diagnostic kit; Arcobacter; bacterium detection; denaturing gradient gel electrophoresis; DNA sequence; entomopathogenic bacterium; environmental health; gene amplification; intermethod comparison; microbial community; microbial consortium; molecular cloning; polymerase chain reaction; restriction fragment length polymorphism; risk assessment; sequence analysis; soil pollution; water contamination; amplified fragment length polymorphism; bacterium; bioreactor; comparative study; denaturing gradient gel electrophoresis; diagnostic kit; high throughput sequencing; isolation and purification; restriction fragment length polymorphism; Arcobacter; Bacteria (microorganisms); Amplified Fragment Length Polymorphism Analysis; Bacteria; Bioreactors; Denaturing Gradient Gel Electrophoresis; High-Throughput Nucleotide Sequencing; Microbial Consortia; Polymorphism, Restriction Fragment Length; Reagent Kits, Diagnostic; Sequence Analysis, DNA
Abstract
Publication date
In
LanguageEnglish
Peer reviewedYes
NPARC number21276961
Export citationExport as RIS
Report a correctionReport a correction (opens in a new tab)
Record identifierb3c31b02-5bce-4104-bad0-5d8233f0cd2b
Record created2015-11-10
Record modified2020-04-22
Date modified: