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Structural characterization of the O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) which possesses human blood group B activity

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/o92-007
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FormatText, Article
SubjectSalmonella milwaukee; lipopolysaccharide; blood group B; polysaccharide; nuclear magnetic resonance
Abstract
The O-polysaccharide of the lipopolysaccharide produced by Salmonella milwaukee O:43 (group U) was shown by composition analysis, methylation, periodate oxidation and 1H and 13C nuclear magnetic resonance spectroscopic analytical methods to be a polymer of branched pentasaccharide repeating units having the structure. The blood-group activity of the O-polysaccharide was established by its serological reactivity with a specific monoclonal antibody to human blood group B, using passive hemaglutination and ELISA assays, indicating the common antigenic epitope to be a nonreducing terminal trisaccharide unit composed of L-Fucp and D-Galp (1:2) residues.
Publication date
PublisherNational Research Council of Canada. NRC Research Press
In
  • Biochemistry and Cell Biology 70, no. 1: 49–55.
LanguageEnglish
Peer reviewedYes
NRC numberPERRY1992A
NPARC number9368602
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Record identifierad339f00-2f5c-4d19-8a4c-ba5e8885b973
Record created2009-07-10
Record modified2023-01-05
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