| Download | - View accepted manuscript: Isolation of Tissue Mast Cells (PDF, 547 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1002/0471142735.im0725s90 |
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| Author | Search for: Kulka, Marianna1; Search for: Metcalfe, Dean D. |
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| Affiliation | - National Research Council of Canada. NRC Institute for Nutrisciences and Health
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| Format | Text, Article |
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| Subject | mast cells; human mast cells; lung; skin; fluorescent activated cell sorting; (FACS); magnetic bead columns |
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| Abstract | Located primarily in tissues, mast cells are one of the principal effector cells in allergic inflammation. Mast cells derive from mononuclear precursor cells which undergo their final phase of differentiation in the tissues. Mast cells express a unique set of proteases and display functional diversity depending on the tissue in which they differentiate—a phenomenon often referred to asmast cell heterogeneity. Enzymatic digestion and density centrifugation have often been used to isolate human mast cells from tissues such as lung and skin, frequently resulting in cells with low viability and purity. Here, we describe a protocol that combines gentle enzymatic digestion with positive selection techniques to isolate reasonably viable and substantially enriched preparations of tissue mast cells. |
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| Publication date | 2010-08-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 17326684 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | aab9806e-33d1-4bfd-afe1-aa8296ced0ed |
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| Record created | 2011-03-18 |
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| Record modified | 2020-04-17 |
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