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Cobalt and sodium requirements for methanogenesis in washed cells of Methanosaeta concilii

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1139/m91-016
AuthorSearch for: 1; Search for: 1
Affiliation
  1. National Research Council of Canada
FormatText, Article
Abstract
Methanosaeta concilii GP6 lost methanogenic activity upon thorough washing of the cells with an anaerobic buffer. Methane production from acetate could be restored by supplementing the washed cell suspension with spent growth medium, and to a lesser degree (65–70% of the rate obtained with spent medium) by the coaddition of NaCl and CoCl2. The latter reactivation had an apparent Km for CoCl2 of 5.6 μM in the presence of 40 mM NaCl, and an apparent Km for NaCl of 2.5 mM in the presence of 5 μM CoCl2. The requirement for NaCl could be met by LiCl but only partially by MgCl2, CaCl2, or NiCl2. Methylcobalamin could substitute for CoCl2. Severe inhibition of methanogenesis in unwashed GP6 cells caused by 5 μM propyl iodide and the role of methylcobalamin in regeneration of methane production in washed cells suggest the involvement of corrinoids in the methane pathway of the aceticlastic Methanosaeta concilii. This represents the first documentation for cobalt/methylcobalamin and sodium requirements for methanogenesis in Methanosaeta concilii. Key words: cobalt, sodium, methylcobalamin, methanogenesis, Methanosaeta concilii.
Publication date
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  • Canadian Journal of Microbiology 37 (1991): 110–115.
LanguageEnglish
NRC numberPATEL1991A
NRCC 31928
NPARC number9365239
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