| DOI | Resolve DOI: https://doi.org/10.1007/s12033-013-9653-9 |
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| Author | Search for: Girard, A.; Search for: Roques, E.; Search for: St-Louis, M.-C.; Search for: Massie, B.1; Search for: Archambault, D. |
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| Affiliation | - National Research Council of Canada
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| Format | Text, Article |
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| Subject | Immunogenicity; Neutralizing antibodies; Replicating but non disseminating adenovectors; Rotaviruses; VP4; VP7; Amino acids; Antibodies; Vaccines; Vectors; Viruses; Mammals; adenovirus vector; chimeric protein; immunoglobulin A; immunoglobulin G; plasmid DNA; protein VP4; protein VP7; virus fusion protein; animal experiment; female; human cell; Human rotavirus; immune response; immunogenicity; lymphocyte; lymphocyte proliferation; mammal cell; mouse; nucleotide sequence; protein expression; virus expression; Adenoviridae; Analysis of Variance; Antibodies, Neutralizing; Antigens, Viral; Capsid Proteins; Cell Line, Tumor; Female; Genetic Vectors; Mice; Mice, Inbred BALB C; Recombinant Fusion Proteins; Rotavirus; Rotavirus Infections; Rotavirus Vaccines; Transfection; Adenoviridae; Human rotavirus A; Mammalia; Mus; Rotavirus |
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| Abstract | The aim of this study was to evaluate the usefulness of replicating but non disseminating adenovirus vectors (AdVs) as vaccine vector using human rotavirus (HRV) as a model pathogen. HRV VP7, VP4, or VP4Δ (N-terminal 336 amino acids of VP4) structural proteins as well as the VP4Δ::VP7 chimeric fusion protein were expressed in mammalian cells when delivered with the AdVs. A preliminary experiment demonstrated that VP4Δ was able to induce a HRV-specific IgG response in BALB/c mice inoculated intramuscularly with AdVs expressing the rotaviral protein. Moreover, an AdV-prime/plasmid DNA-boost regimen of vectors resulted in VP4Δ-specific antibody (Ab) titers ~4 times higher than those obtained from mice immunized with AdVs alone. Subsequently, the various HRV protein-encoding AdVs were compared using the AdV-prime/plasmid DNA-boost regimen. Higher IgG and IgA responses to HRV were obtained when VP4Δ::VP7 fusion protein was used as an immunogen as compared to VP7 or VP4 alone or to a mix of both proteins delivered independently by AdVs. A synergetic effect in terms of Ab was obtained with VP4Δ::VP7. In conclusion, this study demonstrated for the first time the suitability of using replicating but non disseminating AdVs as vaccine vector and the VP4Δ::VP7 fusion protein as an immunogen for vaccination against HRV. © 2013 Springer Science+Business Media New York. |
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| Publication date | 2013 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270683 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a90ed368-b8c5-4901-bb19-655a1fb26f74 |
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| Record created | 2014-02-17 |
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| Record modified | 2020-04-22 |
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