| DOI | Resolve DOI: https://doi.org/10.1166/jnn.2014.9076 |
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| Author | Search for: Fatehi, D.1; Search for: Baral, T.N.1; Search for: Abulrob, A.1 |
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| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
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| Format | Text, Article |
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| Subject | EGFR protein, mouse; epidermal growth factor receptor; monoclonal antibody; quantum dot; brain tumor; fluorescence microscopy; glioblastoma; infrared radiation; metabolism; methodology; molecular imaging; mouse; nude mouse; pathology; reproducibility; sensitivity and specificity; tumor cell line; Antibodies, Monoclonal; Brain Neoplasms; Cell Line, Tumor; Glioblastoma; Infrared Rays; Mice; Mice, Nude; Microscopy, Fluorescence; Molecular Imaging; Quantum Dots; Receptor, Epidermal Growth Factor; Reproducibility of Results; Sensitivity and Specificity |
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| Abstract | Diagnosis of glioblastoma multiform (GBM) with MRI lacks molecular information and requires a biopsy for pathologic confirmation. The EGFRvIII, is a constitutively active mutant of the EGF receptor, identified in a high percentage of brain cancers and associated with increased invasiveness and resistance, making it a good target to improve imaging and diagnosis. The present study shows that conjugation of near-infrared quantum dot (Qd800) to an anti-EGFRvIII single domain antibody, made of the variable region with an extra cysteine for site-specific conjugation (EG2-Cys), increased its internalization in U87MG-EGFRvIII cells in vitro compared to Qd800 conjugated with the Fc region of the antibody (EG2-hFc) or unconjugated. EG2-Cys also improved the contrast in Near-Infrared Imaging of mice bearing orthotopic glioblastoma. The increased accumulation was confirmed by fluorescence microscopy of brain sections. The specificity of EG2-Cys in brain tumor expressing the EGFRvIII mutant receptor may provide an accurate less invasive diagnosis and determine the level of tumor aggressiveness and resistance. Copyright © 2014 American Scientific Publishers All rights reserved. |
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| Publication date | 2014 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-HHT-53273 |
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| NPARC number | 21272265 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | a5e45cec-5d76-4f0a-b0d3-a5823f1821ff |
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| Record created | 2014-07-23 |
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| Record modified | 2020-04-22 |
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