Download | - View accepted manuscript: Derivatives of thiocolchicine and its applications to CEM cells treatment using 19F Magnetic Resonance ex vivo (PDF, 698 KiB)
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DOI | Resolve DOI: https://doi.org/10.1016/j.bioorg.2009.10.002 |
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Author | Search for: Bartusik, Dorota1; Search for: Tomanek, Boguslaw1; Search for: Lattova, Erika; Search for: Perreault, Hélène; Search for: Tuszynski, Jack; Search for: Fallone, Gino |
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Affiliation | - National Research Council of Canada. NRC Institute for Biodiagnostics
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Format | Text, Article |
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Subject | Thiocolchicine; Human T-Lymphoblastoid; Three-dimensional cell culture; Magnetic Resonance Imaging; High Performance Liquid Chromatography coupled with Ultra Violet |
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Abstract | It was shown, that cultured ex vivo human T-Lymphoblastoid (CEM) cells respond to synthesized thiocolchicine and fluorine thiocolchicine derivatives. The preparation of derivatives with substitution at C-3 and C-7 is described. All compounds were used at concentration from 1 nM to 1000 nM. Inhibitory effects of these compounds were examined in the three-dimensional (3-D) culture and cells morphology during treatment was monitored using 9.4 T MRI system. We performed studies of these compounds in CEM cells ex vivo using 1H and 19F Magnetic Resonance Imaging (MRI), 19F Magnetic Resonance Spectroscopy (MRS), High Performance Liquid Chromatography coupled with Ultra Violet (HPLC-UV) and Electron Impact Mass Spectrometry (EIMS). The results of the multi-technique approach are consistent with the fact that the new derivatives are more efficient than colchicine and thiocolchicine ex vivo. |
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Publication date | 2010-02-01 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NPARC number | 17653081 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 9601f55c-c537-459c-bc67-6ee170a072bf |
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Record created | 2011-04-03 |
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Record modified | 2020-04-17 |
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