Simultaneous near-IR spectroscopy and magnetic resonance imaging to assess cerebral oxygenation and brain water during Hypoxia-Ischemia in 2-week-old rats

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Proceedings titleDiagnostic Optical Spectroscopy in Biomedicine
Series titleProceedings of SPIE; no. 4432
ConferenceDiagnostic Optical Spectroscopy in Biomedicine, June 17, 2001, Munich, Germany
Pages5764; # of pages: 8
Subjectfiber optics; hemoglobin oxygenation; edema; tissue scattering; in vivo spectroscopy
AbstractCerebral near-infrared spectroscopy can potentially probe several parameters related to the onset of stroke and the ensuing tissue damage. One obvious marker of ischemia is cerebral oxygenation, which can be lowered sharply in stroke-affected tissue. Also commonly assessed, though less straightforward to recover, is the redox state of the cytochrome aa3 copper center. Finally, parameters that are in principle available but seldom recovered from in vivo near-IR spectra are changes in water concentration and scattering properties of the tissue. We have evaluated the potential for near-IR spectroscopy to detect relevant changes in cerebral oxygenation, blood volume, water content, and scattering properties in an infant rat stroke model that is well characterized by magnetic resonance imaging methods. The specific aim was to acquire near-IR spectra simultaneously with MR images and to correlate stroke-associated changes detected via these two modalities prior to, during and after a hypoxia-ischemia episode within this stroke model. Presented here are results from the design and testing of a near-IR illumination/detection system that is compatible with an MR imaging system, and the recovery of trends in the near-IR spectra that complement the hypoxic-ischemic changes observed in the MR images. Unexpectedly large intensity changes observed for the in vivo near-IR water absorptions are ascribed to hypoxia-induced variations in effective optical pathlength, suggesting that the water absorptions may prove generally useful as a means to track such changes.
Publication date
AffiliationNational Research Council Canada; NRC Institute for Biodiagnostics
Peer reviewedYes
NRC number1902
NPARC number9742236
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Record identifier8ea13822-66ca-4ef5-a1fb-6673ed2df92a
Record created2009-07-17
Record modified2017-09-13
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