Nuciferine prevents hepatic steatosis and injury induced by a high-fat diet in hamsters

  1. Get@NRC: Nuciferine prevents hepatic steatosis and injury induced by a high-fat diet in hamsters (Opens in a new window)
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Journal titlePLoS ONE
Article numbere63770
Subjectaporphine derivative; cholesterol; cytochrome P450 2E1; fatty acid; high density lipoprotein cholesterol; low density lipoprotein cholesterol; nuciferine; triacylglycerol; tumor necrosis factor alpha; unclassified drug; very low density lipoprotein; adipose tissue; animal experiment; animal model; animal tissue; article; body weight; caloric intake; controlled study; down regulation; drug effect; dyslipidemia; fatty liver; food intake; gene expression; hamster; lipid diet; lipogenesis; lipolysis; liver injury; liver weight; male; metabolic syndrome X; nonhuman; protein secretion; reverse transcription polymerase chain reaction; supplementation; upregulation; Western blotting
AbstractBackground:Nuciferine is a major active aporphine alkaloid from the leaves of N. nucifera Gaertn that possesses anti-hyperlipidemia, anti-hypotensive, anti-arrhythmic, and insulin secretagogue activities. However, it is currently unknown whether nuciferine can benefit hepatic lipid metabolism.Methodology/Principal Findings:In the current study, male golden hamsters were randomly divided into four groups fed a normal diet, a high-fat diet (HFD), or a HFD supplemented with nuciferine (10 and 15 mg/kg·BW/day). After 8 weeks of intervention, HFD-induced increases in liver and visceral adipose tissue weight, dyslipidemia, liver steatosis, and mild necroinflammation in hamsters were analyzed. Nuciferine supplementation protected against HFD-induced changes, alleviated necroinflammation, and reversed serum markers of metabolic syndrome in hamsters fed a HFD. RT-PCR and western blot analyses revealed that hamsters fed a HFD had up-regulated levels of genes related to lipogenesis, increased free fatty acid infiltration, and down-regulated genes involved in lipolysis and very low density lipoprotein secretion. In addition, gene expression of cytochrome P4502E1 and tumor necrosis factor-α were also increased in the HFD group. Nuciferine supplementation clearly suppressed HFD-induced alterations in the expression of genes involved in lipid metabolism.Conclusions/Significance:Nuciferine supplementation ameliorated HFD-induced dyslipidemia as well as liver steatosis and injury. The beneficial effects of nuciferine were associated with altered expression of hepatic genes involved in lipid metabolism. © 2013 Guo et al.
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AffiliationNational Research Council Canada (NRC-CNRC); NRC Institute for Nutrisciences and Health (INH-ISNS)
Peer reviewedYes
NPARC number21269671
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Record identifier846ebc09-ebdf-45e5-b512-d5f06b2408d2
Record created2013-12-13
Record modified2016-05-09
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