| DOI | Resolve DOI: https://doi.org/10.1016/j.indcrop.2013.09.029 |
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| Author | Search for: Xu, Yali1; Search for: Salmi, Jani; Search for: Kloser, Elisabeth; Search for: Perrin, Florence2; Search for: Grosse, Stephan1; Search for: Denault, Johanne3; Search for: Lau, Peter C. K.1 |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
- National Research Council of Canada
- National Research Council of Canada. Automotive and Surface Transportation
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| Format | Text, Article |
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| Subject | Aggregated networks; Endoglucanase treatments; Endoglucanases; Fungal enzymes; Nanocrystalline cellulose; Nanocrystalline cellulose(NCC); Physical pre-treatment; Thermostable enzymes; Aspergillus; Atomic force microscopy; Cellulose derivatives; Cloning; Enzymes; Flax; Hemp fibers; Linen; Yarn; Fibers; assessment method; cellulose; crystallinity; enzyme activity; feasibility study; fungus; height; physicochemical property; temperature effect; Cellulose Derivatives; Endo Enzymes; Fibers; Flax; Glucanase; Linen; Yarn |
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| Abstract | Whereas straw management presents a continuing challenge among farmers, tremendous opportunities may exist within the natural fibers in terms of value- added products or chemicals. This research provides the first assessment and demonstration of feasibility of an enzyme-assisted production of nanocrystalline cellulose (NCC) from hemp and flax fibers. A newly cloned endoglucanase (AoEG), derived from Aspergillus oryzae and characterized to be a thermostable enzyme with a half-life of 50. h at 50. °C was used for the hydrolysis. Atomic force microscopy (AFM) imaging of NCC produced from acid swollen cellulose and flax fibers indicated that they form aggregated network, showing rod-like nanofibrils of about 10. nm in height, and 200. nm in length. The yield of NCC using physical pretreatment only or combined physical-chemical pretreatment was compared. The highest yield of NCC was obtained under the conditions of 300. mg of flax fiber treated by 100. IU enzyme at 50. °C for 24. h after pretreatment of the fibers by sonication-microwave in 2% NaOH solution. |
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| Publication date | 2013-09-22 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 55846 |
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| NPARC number | 21269628 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 821487fd-0e92-4eb0-972e-8bdc630b4e12 |
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| Record created | 2013-12-13 |
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| Record modified | 2020-04-22 |
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