Prokaryotic expression of antibodies

  1. Get@NRC: Prokaryotic expression of antibodies (Opens in a new window)
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Journal titleCancer Metastasis Rev.
Pages501519; # of pages: 19
SubjectAnimals; Antibodies; antibody; Antigen Antibody Complex; Antigen-Antibody Complex; biosynthesis; Canada; complex; COMPLEXES; Escherichia; Escherichia coli; ESCHERICHIA-COLI; EXPRESSION; genetics; Humans; Immunoglobulin Fragments; Immunohistochemistry; immunology; isolation & purification; metabolism; protein; Proteins; Recombinant Proteins; SYSTEM; SYSTEMS; yield
AbstractMaximizing the expression yields of recombinant whole antibodies and antibody fragments such as Fabs, single-chain Fvs and single-domain antibodies is highly desirable since it leads to lower production costs. Various eukaryotic and prokaryotic expression systems have been exploited to accommodate antibody expression but Escherichia coli systems have enjoyed popularity, in particular with respect to antibody fragments, because of their low cost and convenience. In many instances, product yields have been less than adequate and intrinsic and extrinsic variables have been investigated in an effort to improve yields. This review deals with various aspects of antibody expression in E. coli with a particular focus on single-domain antibodies
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AffiliationNRC Institute for Biological Sciences; National Research Council Canada
Peer reviewedNo
NPARC number9358875
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Record identifier7cb59911-bf4d-4594-8406-19c253cb70a2
Record created2009-07-10
Record modified2016-05-09
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