| DOI | Resolve DOI: https://doi.org/10.1007/978-3-642-18713-1_1 |
|---|
| Author | Search for: Macmanus, J. P.1; Search for: Jian, M.1; Search for: Preston, E.1; Search for: Webster, J.1; Search for: Zurakowski, B.1 |
|---|
| Affiliation | - National Research Council of Canada. NRC Institute for Biological Sciences
|
|---|
| Format | Text, Book Chapter |
|---|
| Subject | E2F1 transcription factor; focal ischemia; infarct-apoptosis; microtubule; microtubule-associated protein 2 |
|---|
| Abstract | Since cultured neurons which are deficient in the transcription factor E2F1 display a resistance to a wide variety of insults, studies were undertaken to see if this resistance extended to a cerebral ischemic insult. Following 2 h of left middle cerebral artery occlusion and 1 d of reperfusion a 33% smaller infarct (p <0.05) was observed by 2,3,5 triphenyltetrazolium staining in the brains of E2F1-null animals compared to E2F1 and littermate mice. No differences in physiological parameters or cerebrovasculature were observed in the three groups of animals. A milder ischemic insult produced by 20 min of MCA occlusion and 7 d of reperfusion produced a greater difference in the E2F1-null animals with a 71% smaller infarct (p<0.001) compared to littermate controls. A decrease in ischemic injury in E2F1-null mice was also observed by immunohistochemical monitoring of the neuronal-specific MAP2 cytoskeletal protein and TUNEL staining. Finally a battery of six tests of motor function showed both E2F1 and -/littermates were similarly impaired at 1 d of reperfusion, but by 7 d the E2F1 -/mice improved significantly better compared to the wild type mice (p < 0.01) and ended up only slightly impaired by that time. It is concluded that the transcription factor E2F1 does modulate neuronal viability in brain after cerebral ischemia. |
|---|
| Publication date | 2004 |
|---|
| Publisher | Springer |
|---|
| In | |
|---|
| Language | English |
|---|
| Peer reviewed | Yes |
|---|
| NRC number | MACMANUS2002 |
|---|
| Export citation | Export as RIS |
|---|
| Report a correction | Report a correction (opens in a new tab) |
|---|
| Record identifier | 7829b579-a87e-467d-b25e-ce989cb919e5 |
|---|
| Record created | 2009-07-10 |
|---|
| Record modified | 2020-06-12 |
|---|
