| DOI | Resolve DOI: https://doi.org/10.1016/j.phytochem.2014.02.004 |
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| Author | Search for: Banskota, A.H.1; Search for: Stefanova, R.1; Search for: Sperker, S.1; Search for: Lall, S.P.1; Search for: Craigie, J.S.1; Search for: Hafting, J.T.; Search for: Critchley, A.T. |
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| Affiliation | - National Research Council of Canada. Aquatic and Crop Resource Development
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| Format | Text, Article |
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| Subject | Murinae; Palmaria palmata; Palmariaceae; Rhodymenia palmata |
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| Abstract | The EtOAc soluble fraction of a MeOH/CHCl3 extract of Palmaria palmata showed strong nitric oxide (NO) inhibitory activity against lipopolysaccharide (LPS)-induced NO production in murine RAW264.7 cells. NO inhibition-guided isolation led to identification of three new polar lipids including a sulfoquinovosyl diacylglycerol (SQDG) (2S)-1-O-eicosapentaenoyl-2-O- myristoyl-3-O-(6-sulfo-α-d-quinovopyranosyl)-glycerol (1) and two phosphatidylglycerols, 1-O-eicosapentaenoyl-2-O-trans-3-hexadecenoyl-3-phospho- (1′-glycerol)-glycerol (3) and 1-O-eicosapentaenoyl-2-O-palmitoyl-3- phospho-(1′-glycerol)-glycerol (4) from the EtOAc fraction. Seven known lipids were also isolated including a SQDG (2), a phospholipid (5) and five galactolipids (6-10). Structures of the isolated lipids were elucidated by spectral analyses. The isolated SQDGs, phosphatidylglycerols and phospholipid possessed strong and dose-dependent NO inhibitory activity compared to N G-methyl-l-arginine acetate salt (l-NMMA), a well-known NO inhibitor used as a positive control. Further study suggested that these polar lipids suppressed NO production through down-regulation of inducible nitric oxide synthase (iNOS). |
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| Publication date | 2014 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRC-ACRD-55484 |
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| NPARC number | 21272134 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 781eae11-4712-4f65-a2b5-455ddb66577e |
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| Record created | 2014-07-23 |
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| Record modified | 2020-04-22 |
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