Planar patch clamp for neuronal networks - Considerations and future perspectives

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Journal titleMethods in Molecular Biology
Pages93113; # of pages: 21
Subjectsilicon dioxide; silicon nitride; cell adhesion; cell aggregation; immunofluorescence microscopy; microelectromechanical system; microfluidics; microtechnology; nerve cell culture; nerve cell network; nerve cell plasticity; planar patch clamp; scanning electron microscopy
AbstractThe patch-clamp technique is generally accepted as the gold standard for studying ion channel activity allowing investigators to either "clamp" membrane voltage and directly measure transmembrane currents through ion channels, or to passively monitor spontaneously occurring intracellular voltage oscillations. However, this resulting high information content comes at a price. The technique is labor-intensive and requires highly trained personnel and expensive equipment. This seriously limits its application as an interrogation tool for drug development. Patch-clamp chips have been developed in the last decade to overcome the tedious manipulations associated with the use of glass pipettes in conventional patch-clamp experiments. In this chapter, we describe some of the main materials and fabrication protocols that have been developed to date for the production of patch-clamp chips. We also present the concept of a patch-clamp chip array providing high resolution patch-clamp recordings from individual cells at multiple sites in a network of communicating neurons. On this chip, the neurons are aligned with the aperture-probes using chemical patterning. In the discussion we review the potential use of this technology for pharmaceutical assays, neuronal physiology and synaptic plasticity studies.
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PublisherSpringer International Publishing
AffiliationNational Research Council Canada; Human Health Therapeutics; Information and Communication Technologies
Peer reviewedYes
NPARC number21275445
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Record identifier717d87d9-de64-423d-8916-da20da1ea5ec
Record created2015-07-14
Record modified2016-05-09
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