DOI | Resolve DOI: https://doi.org/10.1016/0006-291X(86)90578-4 |
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Author | Search for: Narang, S.; Search for: Dubuc, G.; Search for: Yao, F.; Search for: Michniewicz, J. |
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Format | Text, Article |
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Abstract | Without prior invitro enzymatic ligation a DNA duplex was assembled successfully by directly transforming competent cells with a mixture containing six synthetic complimentary oligodeoxyribonucleotides and a linearized plasmid. One out of 100 transformants was positive in colony hybridization with one of the synthetic fragment probe. The sequence of the DNA duplex inserted into the plasmid was confirmed by dideoxy sequencing method. |
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Publication date | 1986-01-14 |
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Publisher | Elsevier |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NRC number | NARANG1986 |
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NPARC number | 9359486 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 6d9d4c13-b3cb-446a-9ca0-bab8c3be7f84 |
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Record created | 2009-07-10 |
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Record modified | 2020-03-17 |
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