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Application of single-domain antibodies in tumor histochemistry

From National Research Council Canada

DOIResolve DOI: https://doi.org/10.1007/978-1-61779-968-6-33
AuthorSearch for: ; Search for: 1
Affiliation
  1. National Research Council of Canada. NRC Institute for Biological Sciences
FormatText, Article
Subjectcarcinoembryonic antigen related cell adhesion molecule 6; nanobody; pentabody ES1; unclassified drug; antibody specificity; antigen expression; cancer tissue; embedding; human tissue; immunohistochemistry; immunoreactivity; lung carcinoma; procedures; sensitivity and specificity; tissue microarray; tissue section; cytochemistry; metabolism; methodology; neoplasm; pathology; Histocytochemistry; Neoplasms; Single-Domain Antibodies
Abstract
High avidity, pentameric, single-domain antibodies, oligomerized through the B subunit of verotoxin, are excellent immunohistochemical reagents. The resulting molecules are termed pentabodies. Here, we describe the immunostaining of tissue sections with ES1, a pentabody recognizing CEACAM6 which is overexpressed in several cancers. The advantages of pentabodies, compared to conventional antibody reagents, in immunohistochemical studies are highlighted. © 2012 Springer Science+Business Media, LLC.
Publication date
In
  • Methods in Molecular Biology 911 (2012): 523–530.
LanguageEnglish
Peer reviewedYes
NPARC number21269479
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Record identifier6d43d3cb-6420-4f2a-a180-435d6c3f62c8
Record created2013-12-12
Record modified2020-04-21
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