| Download | - View accepted manuscript: A microspore embryogenesis protocol for Camelina sativa, a multi-use crop (PDF, 1.2 MiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s11240-011-9948-0 |
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| Author | Search for: Ferrie, A. M. R.1; Search for: Bethune, T. D.1 |
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| Affiliation | - National Research Council of Canada. NRC Plant Biotechnology Institute
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| Format | Text, Article |
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| Subject | Camelina sativa; doubled haploidy; haploids; microspore culture; microspore embryogenesis |
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| Abstract | Camelina [Camelina sativa (L.) Crantz], a member of the Brassicaceae family, has a unique oil profile that has potential both for biofuels and as a food crop. It is essential to have a doubled haploidy protocol in order to enhance breeding of this crop for prairie conditions as well as improve the yield and quality characteristics, Microspore-derived embryos have been produced from Camelina sativa. Buds 1 – 3 mm in length were selected for culture. The microspores were isolated and purified in full-strength B5 extraction medium and cultured in NLN medium with 12.5% sucrose and 12.5% polyethylene glycol 4000 (PEG) without glutamine, at a density of 10,000 microspores per mL. Glutamine was added to the cultures 72 h after extraction to give a final concentration of 0.8 g/L. The microspore cultures were maintained at 24°C in the dark. After 28 days embryos were observed and these were regenerated to plants and selfed seed was produced. The highest embryogenic frequency achieved was 38 microspore-derived embryos from 100,000 microspores. |
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| Publication date | 2011-04-13 |
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| In | |
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| Note | Erratum published in Volume 107, Issue 2, page 371, November 2011. DOI: 10.1007/s11240-011-0025-5 |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 50179 |
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| NPARC number | 19366509 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 6b417a6d-d6fd-475e-97f2-6480c375f7f2 |
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| Record created | 2012-04-02 |
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| Record modified | 2020-04-21 |
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