Barrier activity in Candida albicans mediates pheromone degradation and promotes mating

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Pages907918; # of pages: 12
SubjectAspartic Endopeptidases; Base Sequence; Candida; Candida albicans; Cell Shape; Cells; cytology; Endopeptidases; Gene Deletion; Genes,Mating Type,Fungal; genetics; Humans; metabolism; Molecular Sequence Data; Mutation; Peptides; pha; Pheromones; physiology; Protease; Protein; Proteins; Receptors,Mating Factor; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; secretion
AbstractMating in Candida albicans and Saccharomyces cerevisiae is regulated by the secretion of peptide pheromones that initiate the mating process. An important regulator of pheromone activity in S. cerevisiae is barrier activity, involving an extracellular aspartyl protease encoded by the BAR1 gene that degrades the alpha pheromone. We have characterized an equivalent barrier activity in C. albicans and demonstrate that the loss of C. albicans BAR1 activity results in opaque a cells exhibiting hypersensitivity to alpha pheromone. Hypersensitivity to pheromone is clearly seen in halo assays; in response to alpha pheromone, a lawn of C. albicans Deltabar1 mutant cells produces a marked zone in which cell growth is inhibited, whereas wild-type strains fail to show halo formation. C. albicans mutants lacking BAR1 also exhibit a striking mating defect in a cells, but not in alpha cells, due to overstimulation of the response to alpha pheromone. The block to mating occurs prior to cell fusion, as very few mating zygotes were observed in mixes of Deltabar1 a and alpha cells. Finally, in a barrier assay using a highly pheromone-sensitive strain, we were able to demonstrate that barrier activity in C. albicans is dependent on Bar1p. These studies reveal that a barrier activity to alpha pheromone exists in C. albicans and that the activity is analogous to that caused by Bar1p in S. cerevisiae
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AffiliationNRC Biotechnology Research Institute; National Research Council Canada
Peer reviewedNo
NRC number47559
NPARC number3540299
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Record identifier68536d1a-0f81-4a55-a785-2d76862b74a6
Record created2009-03-01
Record modified2016-05-09
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