Download | - View accepted manuscript: Decolorization of malachite green by cytochrome c in the mitochondria of the fungus Cunninghamella elegans (PDF, 1002 KiB)
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DOI | Resolve DOI: https://doi.org/10.1016/j.abb.2009.11.027 |
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Author | Search for: Kim, Yong-Hak; Search for: Lee, Cheolju; Search for: Go, Hayoung; Search for: Konishi, Kyoko1; Search for: Lee, Kangseok; Search for: Lau, Peter C. K.1; Search for: Yu, Myeong-Hee |
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Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
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Format | Text, Article |
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Subject | Malachite green; Leucomalachite green; Triphenylmethane dyes; Cytochrome c; Cunninghamella elegans |
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Abstract | We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was determined by optical absorbance spectroscopy with an extinction coefficient (E₅₅₀–₅₃₅) of 19.7 ± 6.3 mM-¹ cm-¹ and reduction potential of + 261 mV. When purified CeCyt was added into the mitochondria, the specific activity of CeCyt reached 440 ± 122 μmol min-¹ mg-¹ protein. The inhibition of MG reduction by stigmatellin, but not by antimycin A, indicated a possible linkage of CeCyt activity to the Qo site of the bc1 complex. The RT-PCR results showed tight regulation of the cecyt gene expression by reactive oxygen species. We suggest that CeCyt acts as a protein reductant for MG under oxidative stress in a stationary or secondary growth stage of this fungus. |
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Publication date | 2010-02-15 |
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Language | English |
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Peer reviewed | Yes |
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NRC number | NRCC 50008 |
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NPARC number | 14262109 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 6496d10b-f81e-44bd-b0b5-53afd32c457a |
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Record created | 2010-06-02 |
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Record modified | 2020-04-17 |
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