| Download | - View accepted manuscript: The preparation of certified calibration solutions for azaspiracid-1, -2, and -3, potent marine biotoxins found in shellfish (PDF, 678 KiB)
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| DOI | Resolve DOI: https://doi.org/10.1007/s00216-010-4161-2 |
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| Author | Search for: Perez, Ruth A.1; Search for: Rehmann, Nils; Search for: Crain, Sheila1; Search for: LeBlanc, Patricia1; Search for: Craft, Cheryl1; Search for: MacKinnon, Shawna1; Search for: Reeves, Kelley1; Search for: Burton, Ian W.1; Search for: Walter, John A.1; Search for: Hess, Philipp; Search for: Quilliam, Michael A.1; Search for: Melanson, Jeremy E. |
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| Affiliation | - National Research Council of Canada. NRC Institute for Marine Biosciences
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| Format | Text, Article |
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| Subject | Reference materials; Mass spectrometry/LC-MS; Marine toxins |
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| Abstract | The production and certification of a series of azaspiracid (AZA) calibration solution reference materials is described. Azaspiracids were isolated from contaminated mussels, purified by preparative liquid chromatography and dried under vacuum to the anhydrous form. The purity was assessed by liquid chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy. The final concentration of each AZA in a CD 3OH stock solution was determined by quantitative NMR spectroscopy. This solution was then diluted very accurately in degassed, high purity methanol to a concentration of 1.47 ± 0.08 μmol/L for CRM-AZA1, 1.52 ± 0.05 μmol/L for CRM-AZA2, and 1.37 ± 0.13 μmol/L for CRM-AZA3. Aliquots were dispensed into argon-filled glass ampoules, which were immediately flame-sealed. The calibration solutions are suitable for method development, method validation, calibration of liquid chromatography or mass spectrometry instrumentation and quality control of shellfish monitoring programs. |
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| Publication date | 2010-11-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NRC number | NRCC 51766 |
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| NPARC number | 12745771 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 5675642f-2b1a-4196-ba4f-22f72c4f0e03 |
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| Record created | 2009-10-28 |
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| Record modified | 2020-04-17 |
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