Targeted MRI and optical molecular imaging using gadolinium loaded small unilamellar vesicles

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Proceedings titleAIChE Annual Meeting, Conference Proceedings
Conference2011 AIChE Annual Meeting, 11AIChE, 16 October 2011 through 21 October 2011, Minneapolis, MN
SubjectAnti-EGFR; Brain tumor model; Brain tumors; Enhanced sensitivity; Functionalized; In-vitro; In-vivo; Key component; Molecular process; MRI contrast agents; Nano-sized; Near-infrared dyes; Novel techniques; Optical imaging; Optical molecular imaging; Single domains; Small unilamellar vesicle; Targeted contrast agents; Unique features; Antibodies; Brain; Dynamic light scattering; Gadolinium; Magnetic resonance imaging; Optical image storage; Synchrotrons; Molecular imaging
AbstractNoninvasive investigation of cellular and molecular processes becomes possible through the novel techniques, one of which is molecular imaging, where enhanced sensitivity is a key component for clinic translation of the technique. In this presentation, spontaneously forming, small unilamellar vesicles (ULVs) (30 nm in diameter) were used as a platform to build a bi-modal [i.e., optical and Magnetic Resonance Imaging (MRI)] targeted contrast agent for the molecular imaging of brain tumors. Small ULVs were loaded with a gadolinium (Gd) chelated lipid (Gd-DPTA-BOA), functionalized with targeting antibodies (anti-EGFR monoclonal and anti-IGFBP7 single domain), and incorporated a near infrared dye (Cy5.5). The resultant ULVs were characterized in vitro using small angle neutron scattering (SANS), in phantom MRI and dynamic light scattering (DLS). Targeted (with antibodies) and nontargeted-Gd loaded sULVs labeled with Cy5.5 were assessed in vivo in a mice brain tumor model using both optical imaging and MRI. The results demonstrated that a spontaneously forming, nanosized ULV loaded with a high payload of Gd can selectively target and image, using MR and optical imaging, brain tumor vessels when functionalized with antibodies. The unique features of these targeting ULVs make them promising molecular MRI contrast agents.
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AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21271701
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Record identifier42dd5adb-cc1d-4003-9191-b8578df2bb88
Record created2014-03-24
Record modified2016-05-09
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