Structural studies of the cell wall polysaccharides from three strains of Lactobacillus helveticus with different autolytic properties: DPC4571, BROI, and LH1

From National Research Council Canada

DOI	Resolve DOI: https://doi.org/10.1016/j.carres.2013.05.020
Author	Search for: Vinogradov, E.¹; Search for: Valence, F.; Search for: Maes, E.; Search for: Jebava, I.; Search for: Chuat, V.; Search for: Lortal, S.; Search for: Grard, T.; Search for: Guerardel, Y.; Search for: Sadovskaya, I.
Affiliation	National Research Council of Canada
Format	Text, Article
Subject	2D-NMR spectroscopy; Autolytic properties; Cell wall polysaccharides; Cell-wall components; Intracellular enzyme; Lactobacillus helveticus; Methylation analysis; Structural studies; Alkylation; Antigen-antibody reactions; Cells; Enzymatic hydrolysis; Nuclear magnetic resonance spectroscopy; Polysaccharides; Strain; Structure (composition); Cytology; autolysin; peptidoglycan; polysaccharide; teichoic acid; autolysis; bacterial strain; cell wall; chemical structure; hydrolysis; Lactobacillus helveticus; methylation; nuclear magnetic resonance spectroscopy; protein structure; Lactobacillus helveticus
Abstract	Lactobacillus helveticus is traditionally used in dairy industry as a starter or an adjunct culture for manufacture of cheese and some types of fermented milk. Its autolysis releases intracellular enzymes which is a prerequisite for optimum cheese maturation, and is known to be strain dependent. Autolysis is caused by an enzymatic hydrolysis of the cell wall peptidoglycan (PG) by endogenous peptidoglycan hydrolases (PGHs) or autolysins. Origins of differences in autolytic properties of different strains are not fully elucidated. Regulation of autolysis possibly depends on the structure of the cell wall components other than PG, particularly polysaccharides. In the present work, we screened six L. helveticus strains with different autolytic properties: DPC4571, BROI and LH1. We established, for the first time, that cell walls (CWs) of these strains contained polysaccharides, different from their CW teichoic acids. Cell wall polysaccharides of three strains were purified, and their chemical structures were established by 2D NMR spectroscopy and methylation analysis. The structures of their repeating units are presented. © 2013 Elsevier Ltd.
Publication date	2013
In	Carbohydrate Research 379 (2013): 7–12.
Language	English
Peer reviewed	Yes
NPARC number	21270591
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Record identifier	41b1fb8c-f014-4077-8ab1-a34915352453
Record created	2014-02-17
Record modified	2020-04-22

Date modified:: 2024-04-18