DOI | Resolve DOI: https://doi.org/10.1159/000348744 |
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Author | Search for: Khan, W.A.; Search for: Chisholm, R.1; Search for: Tadayyon, S.; Search for: Subasinghe, A.; Search for: Norton, P.; Search for: Samarabandu, J.; Search for: Johnston, L.J.1; Search for: Knoll, J.H.; Search for: Rogan, P.K. |
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Affiliation | - National Research Council of Canada. NRC Steacie Institute for Molecular Sciences
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Format | Text, Article |
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Subject | centromere protein B; satellite DNA; atomic force microscopy; centromere; comparative study; DNA sequence; fluorescence microscopy; fluorescent lighting; human cell; human chromosome; metaphase chromosome; protein localization; Cell Line, Tumor; Centromere; Centromere Protein B; Chromatin; Chromosomes, Human, Pair 17; DNA Probes; DNA, Satellite; Fluorescent Antibody Technique; In Situ Hybridization, Fluorescence; Lymphocytes; Metaphase; Microscopy, Atomic Force; Tissue Fixation |
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Abstract | Despite extensive analyses on the centromere and its associated proteins, detailed studies of centromeric DNA structure have provided limited information about its topography in condensed chromatin. We have developed a method with correlative fluorescence light microscopy and atomic force microscopy that investigates the physical and structural organization of -satellite DNA sequences in the context of its associated protein, CENP-B, on human metaphase chromosome topography. Comparison of centromeric DNA and protein distribution patterns in fixed homologous chromosomes indicates that CENP-B and -satellite DNA are distributed distinctly from one another and relative to observed centromeric ridge topography. Our approach facilitates correlated studies of multiple chromatin components comprising higher-order structures of human metaphase chromosomes. © 2013 S. Karger AG, Basel. |
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Publication date | 2013 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NPARC number | 21269955 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 36ff577c-e59c-4f70-b977-cbfca7751a4a |
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Record created | 2013-12-13 |
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Record modified | 2020-10-02 |
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