Rapid and specific SPRi detection of L. pneumophila in complex environmental water samples

  1. Get@NRC: Rapid and specific SPRi detection of L. pneumophila in complex environmental water samples (Opens in a new window)
DOIResolve DOI: http://doi.org/10.1007/s00216-015-8726-y
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Journal titleAnalytical and Bioanalytical Chemistry
SubjectBiosensors; Cooling water; Nucleic acids; Optical sensors; Protozoa; Risk assessment; Surface plasmon resonance; Water quality; Water towers; Environmental conditions; Environmental sample; Environmental water samples; Legionella pneumophila; Nanoparticles/Nanotechnology; Rapid detection systems; Surface plasmon resonance imaging; Water quality assessments; RNA
AbstractLegionellosis is a very devastating disease worldwide mainly due to unpredictable outbreaks in man-made water systems. Developing a highly specific and sensitive rapid detection system that detects only metabolically active bacteria is a main priority for water quality assessment. We previously developed a versatile technique for sensitive and specific detection of synthetic RNA. In the present work, we further investigated the performance of the developed biosensor for detection of Legionella pneumophila in complex environmental samples, particularly those containing protozoa. The specificity and sensitivity of the detection system were verified using total RNA extracted from L. pneumophila in spiked water co-cultured with amoebae. We demonstrated that the expression level of ribosomal RNA (rRNA) is extremely dependent on the environmental conditions. The presence of amoebae with L. pneumophila, especially in nutrition-deprived samples, increased the amount of L. pneumophila 15-fold after 1 week as measured through the expression of 16s rRNA. Using the developed surface plasmon resonance imaging (SPRi) detection method, we were also able to successfully detect L. pneumophila within 3 h, both in the presence and absence of amoebae in the complex environmental samples obtained from a cooling water tower. These findings suggest that the developed biosensing system is a viable method for rapid, real-time and effective detection not only for L. pneumophila in environmental samples but also to assess the risk associated with the use of water contaminated with other pathogens. © 2015 Springer-Verlag Berlin Heidelberg
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AffiliationNational Research Council Canada (NRC-CNRC)
Peer reviewedYes
NPARC number21275742
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Record identifier35c49026-0503-4b27-aa95-ccf92ef81268
Record created2015-07-14
Record modified2016-05-09
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