Use of the zebrafish larvae as a model to study cigarette smoke condensate toxicity

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Journal titlePLoS ONE
Article numbere115305
Subjectcigarette smoke condensate; acute toxicity; animal experiment; animal model; cardiotoxicity; cost effectiveness analysis; developmental toxicity; embryo; high throughput screening; in vivo study; larva; neurotoxicity; smoking; zebra fish; Danio rerio; Mammalia; Nicotiana tabacum
AbstractThe smoking of tobacco continues to be the leading cause of premature death worldwide and is linked to the development of a number of serious illnesses including heart disease, respiratory diseases, stroke and cancer. Currently, cell line based toxicity assays are typically used to gain information on the general toxicity of cigarettes and other tobacco products. However, they provide little information regarding the complex disease-related changes that have been linked to smoking. The ethical concerns and high cost associated with mammalian studies have limited their widespread use for in vivo toxicological studies of tobacco. The zebrafish has emerged as a low-cost, high-throughput, in vivo model in the study of toxicology. In this study, smoke condensates from 2 reference cigarettes and 6 Canadian brands of cigarettes with different design features were assessed for acute, developmental, cardiac, and behavioural toxicity (neurotoxicity) in zebrafish larvae. By making use of this multifaceted approach we have developed an in vivo model with which to compare the toxicity profiles of smoke condensates from cigarettes with different design features. This model system may provide insights into the development of smoking related disease and could provide a cost-effective, high-throughput platform for the future evaluation of tobacco products.
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AffiliationAquatic and Crop Resource Development; National Research Council Canada
Peer reviewedYes
NRC numberNRC-ACRD-55848
NPARC number21275520
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Record identifier2cb6387c-1213-4286-8cd9-515bcef391ad
Record created2015-07-14
Record modified2016-05-09
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