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Solution conformation of asparagine-linked oligosaccharides: α(1-2)-, α(1-3)-, β(1-2)-, and β(1-4)-linked units

From National Research Council Canada

Alternative titleSolution conformation of asparagine-linked oligosaccharides: alpha(1-2)-, alpha(1-3)-, beta(1-2)-, and beta(1-4)-linked units
DOIResolve DOI: https://doi.org/10.1021/bi00284a021
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FormatText, Article
Subject1H; alpha; asparagine; asparagine-linked oligosaccharides; calculation; carbohydrate conformation; carbohydrate sequence; chemical; chemical shift; conformation; conformational; energy; energy calculations; enhancement; glcnac; glycopeptide; high-resolution; kinetics; linkages; magnetic; magnetic resonance spectroscopy; models, molecular; molecule; NMR; nuclear; nuclear magnetic resonance; nuclear-magnetic-resonance; oligosaccharide; oligosaccharides; overhauser; overhauser enhancement; parameters; potential; residues; resonance; shifts; solution; solution conformation; structure-activity relationship; support, Non-U. S. Gov't; unit
Abstract
The solution conformation is presented for representatives of each of the major classes of asparaginyl oligosaccharides. In this report the conformation of α(1-3)-, α(1-2)-, β(1-2)-, and β(1-4)-linked units is described. The conformational properties of these glycopeptides were determined by high-resolution ¹H nuclear magnetic resonance in conjunction with potential energy calculations. The NMR parameters that were used in this analysis were chemical shifts and nuclear Overhauser enhancements. Potential energy calculations were used to evaluate the preferred conformers available for the different linkages in glycopeptides and to draw conclusions about the behavior in solution of these molecules. It was found that the linkage conformation of the Man α 1-3 residues was not affected by substitution either at the 2-position by α Man or β GlcNAc or at the 4-position by beta GlcNAc or by the presence of a bisecting GlcNAc on the adjacent β Man residue
Publication date
PublisherAmerican Chemical Society
In
  • Biochemistry 22, no. 15: 3671–3680.
LanguageEnglish
Peer reviewedYes
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IdentifierNRC-IBS-BRISSON1983G
NPARC number9388957
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Record identifier265902d8-134d-4610-87cf-f21a447bdf6f
Record created2009-07-10
Record modified2020-03-13
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