DOI | Resolve DOI: https://doi.org/10.1021/bc9001147 |
---|
Author | Search for: Boucher, Cyril1; Search for: Liberelle, BenoÎt; Search for: Jolicoeur, Mario; Search for: Durocher, Yves1; Search for: De Crescenzo, Gregory |
---|
Affiliation | - National Research Council of Canada. NRC Biotechnology Research Institute
|
---|
Format | Text, Article |
---|
Subject | Animal; biotechnology; Biotechnology; Epidermal Growth Factor; Phosphorylation; Protein; Proteins; Tissue Engineering |
---|
Abstract | We have elaborated and validated a novel approach for the oriented tethering of proteins such as the epidermal growth factor (EGF) on aminated surfaces. The grafting reactions were optimized to generate a dense and homogeneous EGF layer. Impact of EGF orientation on A-431 cellular response was investigated. Our results demonstrate that, in sharp contrast to responses obtained with soluble EGF supply or with randomly grafted EGF, oriented immobilization of EGF via a de novo designed coiled-coil capture system leads to a sustained phosphorylation of A-431 cell surface EGF receptors. Our results thus indicate that oriented protein immobilization via coiled-coil interactions is an efficient and versatile method to control tethering of bioactive molecules for future applications in the field of regenerative medicine and tissue engineering. |
---|
Publication date | 2009-09-07 |
---|
In | |
---|
Language | English |
---|
Peer reviewed | Yes |
---|
NRC number | NRCC 52736 |
---|
NPARC number | 14021379 |
---|
Export citation | Export as RIS |
---|
Report a correction | Report a correction (opens in a new tab) |
---|
Record identifier | 25695f56-bcf0-4d5b-ab48-f60592a19e6c |
---|
Record created | 2010-06-02 |
---|
Record modified | 2020-04-16 |
---|