| DOI | Resolve DOI: https://doi.org/10.1007/s10529-012-0858-y |
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| Author | Search for: Gul-Uludag, Hilal; Search for: Lu, Weibing; Search for: Xu, Peng1; Search for: Xing, James; Search for: Chen, Jie1 |
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| Affiliation | - National Research Council of Canada. Security and Disruptive Technologies
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| Format | Text, Article |
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| Subject | Cancer; Cellular and nuclear uptake; Fluorescein isothiocyanate; Gene therapies; Magnetic carbon nanotubes; Monocytic leukemia cells |
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| Abstract | Monocyte-based gene therapies in cancer have been hampered by either the resistance of these cells to non-viral molecular delivery methods or their poor trafficking to the tumor site after their ex vivo manipulations. Magnetic nanoparticles (MNP)-loaded genetically engineered monocytes can efficiently delivered to tumor site by external magnetic field, but they are not ideal delivery tools due to their spherical shape. Hence, we have investigated the cellular uptake efficiency and cytotoxicity of fluorescein isothiocyanate (FITC)-labelled magnetic carbon nanotubes (FITC-mCNT) in human monocytic leukemia cell line THP-1 for application in cell-based gene therapy against cancer. Uptake of FITC-mCNT into THP-1 cells reached 100% only 1 h after the delivery. Confocal imaging confirmed that FITC-mCNT entered the cell cytoplasm and even into the nucleus. FITC-mCNT uptake did not compromise cell viability. This delivery system might therefore enhance cell-based cancer gene therapies. |
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| Publication date | 2012-05-01 |
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| In | |
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| Language | English |
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| Peer reviewed | Yes |
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| NPARC number | 21270217 |
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| Export citation | Export as RIS |
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| Report a correction | Report a correction (opens in a new tab) |
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| Record identifier | 2265506a-9011-4482-89f5-83a5e75598be |
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| Record created | 2014-01-13 |
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| Record modified | 2020-04-21 |
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