DOI | Resolve DOI: https://doi.org/10.1016/j.biomaterials.2012.10.047 |
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Author | Search for: Fortier, Charles1; Search for: De Crescenzo, Gregory; Search for: Durocher, Yves1 |
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Affiliation | - National Research Council of Canada. Human Health Therapeutics
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Format | Text, Article |
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Subject | transfection; polyethylenimine; non-viral vector; targeting; oriented tethering; epidermal growth factor (EGF) |
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Abstract | Surface modification of non-viral gene delivery nanocarriers may provide advanced features such as receptor targeting, endosomal escape and nuclear import. We here report the design of a versatile and tunable immobilization protocol to functionalize nanocarriers for improved transient gene expression. Our strategy is based on specific interactions occurring between a coil-tagged ligand and a complementary coil-functionalized nanocarrier. As a proof of concept, targeting of DNA/polyethylenimine polyplexes to the epidermal growth factor receptor of A431 cells was investigated. Coiled-coil-mediated oriented tethering of epidermal growth factor triggered a drastic increase of the internalization rate of the targeted polyplexes. To explore the tunability of our platform, surface density of targeting ligand was varied; our results indicated that the internalization rate varied with the ligand-to-polyplex ratio in a “switch mode” fashion. This work prefigures possible avenues for our coiled-coil platform in multiplex functionalization to address transient gene expression bottlenecks in recombinant protein production. |
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Publication date | 2012-11-06 |
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In | |
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Language | English |
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Peer reviewed | Yes |
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NRC number | NRCC 53182 |
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NPARC number | 21268241 |
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Export citation | Export as RIS |
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Report a correction | Report a correction (opens in a new tab) |
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Record identifier | 1c9e37f0-5368-46c5-8d02-fe1ea701247c |
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Record created | 2013-06-07 |
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Record modified | 2020-04-21 |
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